Naphthol AS-D Chloroacetate Esterase (AS–DCE) Stain
Intended Use:
This kit is for staining bone marrow cell and bloodcell smear.
Principle:
Naphthol AS-D chloroacetate can be hydrolyzed into Naphthol AS-D by Esterase to mix with diazonium salt and form insoluble red-brown precipitate in cytoplasm. Positive reaction is only found in granulocytes; hence, it is also called "Specific Esterase Stain".
Specifications:
| Contents | 5Tests/Kit | 20Tests/Kit | 100Tests/Kit | Components |
|
Fixative (solution A) |
1vial×2.5m | 1vial×10ml | 1vial×50ml | Sodium Citrate |
|
Pararosaniline (solution B) |
1vial×0.1ml | 1vial×0.3ml | 1vial×1.1ml | Parafuchsin |
|
Sodium Nitrite (solution C) |
1vial×0.1ml | 1vial×0.3ml | 1vial×1.1ml | Sodium Nitrite |
|
Phosphate Buffer (solution D) |
1vial×5ml | 1vial×20ml | 1vial×95ml | Phosphate |
|
Naphthol As-D Chloroacetate (solution E) |
1vial×0.3ml | 1vial×1.2ml | 1vial×5.5ml |
Naphthol AS-D hloroacetate |
|
Methyl Green (solution F) |
1vial×5ml | 1vial×20ml | 1vial×100ml | Methyl Green |
Methods:
Working solution Preparation（for 1 test only）;
Apparatus required: disposable tube, micropipette, disposable tips, dropper;
Instruction: Mix 10µl solution B and10µl solution C thoroughly. Wait for 1 Minute;
Add 0.9ml solution D and 50µl solution E. Mix well;
Expected Results:
Red granules found in cytoplasm means positive.
