Giemsa Stain

 

Intended Use:

This kit is for staining blood cell and chromosomes.

 

Principle:

Blood Cell Staining: Giemsa Stain is a mixture of methylene blue and eosin.
Methylene blue is alkaline, and eosin is acid. Because each cell and its components are different in chemical composition, their affinities to acid stain and alkaline stain of this kit vary significantly. Consequently, one can  identify cells based on their staining color, shape and other physical characteristics.

 

Methods:

Blood cell staining:

1. Dry the blood smear and fix in Methyl alcohol for 1~3 mins.

2. Place the fixed blood smear in diluted Giemsa stain(solution A: solution B= 1:9) for10~30mins.(Use drop

    stain if specimens are not too many.) At lower room temperature in winter, incubate at 37℃.

3. Take out the slide and rinse with water. Dry the slide for microscopic examination.

 

Chromosome staining:

1. Pretreat the specimen with trypsin.

2. Dip the specimen into diluted Giemsa stain(solutionA: solution B=1:9) for 10 mins.

3. Rinse with water and examine under a microscope after drying the slide.

 

Specifications:

Contents		Components
Giemsa solution A	1vial×100ml	Giemsa stain
Giemsa solution B	4vials×250ml	Giemsa stain

 

Expected Results:

Blood cell staining: Erythrocyte appears vermeil. Leukocyte appears varying shades of blue to dark blue. Structure of nuclei is clear.Cytoplasmic granules are stained clearly and appear the specific color of each cell.